An English hospital on Thursday became the nation's first to win authorisation to use the three-person fertility technique in an effort to prevent inherited disease.
Britain last year became the first country in the world to legally offer such a treatment after parliament approved legislation in December.
Doctors at the Newcastle Fertility Centre in northeast England will however not be able to go ahead with technique until an application by an individual patient has been approved.
"This significant decision represents the culmination of many years hard work by researchers, clinical experts and regulators," said Sally Cheshire, head of the Human Fertilisation and Embryology Authority.
"Patients will now be able to apply individually to the HFEA to undergo mitochondrial donation treatment at Newcastle, which will be life-changing for them, as they seek to avoid passing on serious genetic diseases to future generations," she said.
British lawmakers voted in 2016 to allow the treatment, in which the DNA of the mother, father and a female donor are combined to create a baby.
Mitochondria are structures in cells which generate vital energy and contain their own set of genes called mDNA which is passed through the mother.
Mitochondrial diseases cause symptoms ranging from poor vision to diabetes and muscle wasting, and health officials estimate around 125 babies are born with the mutations in Britain every year.
The first baby conceived using mitochondrial donation was born in 2016 in Mexico, where there are no rules on its use, but Britain is the first to officially authorise it.
Around 3,000 British families could benefit from the therapy, but Cheshire said she expected that "many won't come forward".
The treatment remains controversial in Britain and elsewhere, with religious leaders among its detractors.
The Roman Catholic Church opposes the move, pointing out that it would involve the destruction of human embryos as part of the process, while the Church of England has said ethical concerns "have not been sufficiently explored".
The Newcastle clinic said it was "delighted".
"This announcement gives a hope and silver lining as we all strive together to help these women," said Meenakshi Choudhary, consultant gynaecologist at the clinic.
Adam Balen, chair of the British Fertility Society said it was "a historical step towards eradicating genetic disease".